![]() (1979) Efficient transfer of large DNA fragments from agarose gels to diazobenzloxymethal-paper and rapid hybridization by using dextran sulfate. DuPont Manufacturers Instruction Booklet, Du Pant, Wilmington, DE. This review will focus on the regulation of biofilms by RNA regulatory mechanisms small noncoding RNAs (sRNAs) and ribonucleases (RNases), quorum sensing (QS) and cyclic diguanylate (cdiGMP), all of which are important for all stages of biofilm formation, in particular, to the initial steps. (1989) GeneScreen hybridization transfer membrane UV-crosslinking protocols. (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. F., and Maniatis, T.(1989) Molecular Cloning: A Laboratory Manual. (1972) Purification of biologically active globin mRNA by chromatography on oligothymidylic acid-cellulose. (1985) Developmental regulation of alpha-fetoprotein in transgenic mice. Although Northern blot analysis showed a slight reduction in bap. Both procedures rely on the annealing (hybridization) of complementary single-stranded nucleic acid molecules under suitable conditions (3). (1980) Purification of mouse immuno:globulin heavy-chain RNAs from total myeloma tumor RNA. RT article T1 Extracellular proteases inhibit protein-dependent biofilm formation in. Soon after the introduction of the Southern blotting procedure (1), an analogous technique, known as northern blotting, was developed for the analysis of RNA sequences (2). ![]() (1989) The murine and Drosophila homeobox gene complexes have common features of organization and expression. This study shows that this variation of biofilm formation in S. Graham, A., Papalopulu, N., and Krumlauf, R. The data of Northern blot and complementation showed that SigB plays an. (1988) Characterization of a murine homeobox gene, Hox-2.6, related to the Drosophila deformed gene. Northern blot analysis was performed on total RNA extracts prepared from HG001 strain and an. Graham, A., Papalopulu, N., Lorimer, J., McVey, J. RsaI inhibits the repressor of biofilm formation icaR. Northern blotting and 5-rapid amplification of. Northern blotting can be used to analyze a sample of RNA from a particular tissue or. (1987) Developmental and spatial patterns of expression of the mouse homeobox gene, Hox-2.1. Adjacent downstream genes walK, EF1195, EF1196, and EF1197 were co-transcribed and detect antisense walR RNA. A northern blot is a laboratory method used to detect specific RNA molecules among a mixture of RNA. Tormo, M.A., Ubeda, C., Marti, M., Maiques, E., Cucarella, C., Valle, J., Foster, T.J., Lasa, I., and Penades, J.R.Krumlauf, R., Holland, P. aureus can detach from a biofilm by switching to a Bap-negative state. c Northern blot analysis using sipW and bsaR gene-specific DNA probes. We performed growth analysis, northern blot analysis, and biofilm assays using CDMM supplemented. The absence of Bap correlated with increased adherence to fibrinogen and fibronectin. We also used modified chemically defined medium (CDMM) 12. Streptococcus mutans, Operon and Biofilms ResearchGate, the professional. ![]() Experimental infection of ovine mammary glands with Bap-negative variants showed that phase variation occurred in vivo, because Bap-expressing, biofilm-positive revertants were isolated from infected mammary glands. We show here that inactivation of pdhD, putatively encoding a subunit of the pyruvate dehydrogenase complex, impairs. The biofilm-negative phenotype reverted to wild-type at a similar frequency upon subculture of Bap-negative variants in liquid media. However, DNA typing, Southern blot hybridization and DNA sequencing did not show any differences between the parent V329 strain and the biofilm-negative variants. Northern blot analysis of these variants with a bap-specific gene probe showed that transcription of the bap gene did not occur. aureus strain V329 on Congo red agar, spontaneous smooth biofilm-negative colonies appeared at a low frequency (5×10−4). A process of phase variation is described that affects the expression of Bap (biofilm-associated protein) in Staphylococcus aureus. ![]()
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![]() ![]() Bus stabs without notches do not allow for tandem breakers installation but will allow for non-class CTL tandem breakers (QO) as replacements only. Panelboards with notched bus stabs allow for installation of CTL tandem breakers (QOT). With this code, manufacturers are required to list the number of max number of circuit breakers allowed and to provide a “rejection feature” to help prevent people from installing tandem breakers where they are not allowed to be installed. The 2008 National Electrical Code (NEC) got rid of the “lighting and appliance” designation and now says, “A panelboard shall be provided with physical means to prevent the installation of more overcurrent devices than that number for which the panelboard was designed, rated, and listed.” At various points in time, Square D has labeled tandem circuit breakers with part numbers that did not hold true to this rule.Īll panelboards must adhere to UL standards and included in this requires “lighting and appliance” panelboards to be class CTL. QO (e.g., QO2020) are circuit breakers are designed for use in the older “non-class CTL” QO load centers that were manufactured before the change in code. In general, QOT (e.g., QOT2020) are the new version of tandem breakers designed to be used in present class CTL QO load centers. Horizontal Switch with Hook Horizontal Switch w/o Hook Vertical Switch w/ Hook Class CTL & QO vs QOTĬlass CTL (Circuit Total Limiting) is a classification of load centers from the 1965 National Electrical Code (NEC). Usually if a breaker has a hook it is referred to as a “QOT” and if it does not then it would be considered a “QO Twin or Tandem”, but this is not always the case. Some use a common clip while others use a larger “hook”, which only allows the breakers to be mounted into specific spots on a panel. ![]() ![]() The hook refers to the way the back of the breaker clips into the panel on the bottom side. Vertical (Stack) Switch Horizontal Switch Hook The horizontal version is the newer design. ![]() Vertical units are stacked top to bottom whereas horizontal units have the switches side-by-side. The vertical vs horizontal refers to the switches on the face of the breaker. Let’s learn more about what these mean… Vertical vs Horizontal Here are the four varieties of Square D QO tandem breakers:įrom left to right we have the vertical switch with hook, horizontal switch with hook, horizontal switch without hook, vertical switch without hook. This is a normal and legitimate practice as long as the panelboard is designed for tandem circuit breakers and these breakers are installed on the panelboard where they are specifically allowed. For this reason, they’re usually used on panelboards that has filled to capacity with standard breakers. Some may confuse tandem breakers with two-pole circuit breakers, however, tandem breakers do not get connected to two different poles at a panelboard and do not have a common trip or handle tie for simultaneous disconnecting of two poles - unlike a two-pole circuit breaker.Ī tandem circuit breaker is a double circuit breaker that takes up one circuit breaker space on a panelboard. Tandem breakers are known by different names, such as duplex, slimline, twin, half-height, half-inch, double and wafer breakers. Let’s get things straight once and for all… Tandem Breaker definition The thing is, although many Square D QO tandems have the same part number, they don’t all interchange. What is it about tandem / twin breakers that confuse so many of us? Maybe if they gave these bad boys a more specific part number then it wouldn’t be so bad, right? Need something different? Other sizes, voltages and configurations are available. ![]() ![]() ![]() The original white Barbie Ferrari was showcased in 1988, with the bold, Italian red edition of the Barbie convertible in “Fastback” style hot on its heels and available to buy in 1990. Its iconic hot pink “Barbie” license plate, a trunk that opened and actual Jaguar dashboard detailing made it a must-have in the world of Barbie convertibles. Wrapped in a “dazzling, glittery pink”, the Barbie convertible Jaguar XJS sparkled and shone its way into toy boxes across the nation. Did any of your favorites appear in the list below? ![]() The franchise expanded – or should we say, exploded – in a flurry of luxury vehicles, campervans and boats. Architectural ‘marvels’ worth mentioning were its fully furnished rooms and swing on the front porch.ĩ0s Barbie playsets didn’t stop short at fully-kitted out 90s Barbie houses, oh no. Evolving to reflect current trends and styles typical of the late 90s, this 90s Barbie house was more muted in its exterior appearance but showcased stained glass bay windows, a balcony as well as arches and columns. With working electronics like a ringing telephone and doorbell, the 90s Barbie house also showcases glowing highlights like a fireplace and chandeliers.īarbie upgraded her mortgage in 1998 to a pastel pink Barbie Dreamhouse that was as deluxe as the name suggests. Of all the 90s Barbie playsets a girl could have, the Magical Mansion was lavish and chock full of accessories and upgrades. Sophisticated, colorful and decadent, the Barbie Townhouse even had a fully functional pink elevator. The Barbie Townhouse featured everything “in Vogue” and boasted a far bolder design than its 1974 counterpart. Though technically a product of the 80s, many 90s Barbie fans will remember playing with their older sister’s 80s-Tastic Townhouse before graduating to the Magical Mansion in the early 1990s. And just as homes over the years have changed and evolved over the past four-and-a-half decades, so have these 90s Barbie houses. Made with cardboard and paper so that it could be folded up and carried with you, the original, mid-century Barbie Dreamhouse hit shelves in 1962. ![]() ![]() ![]() Lighter boards can also be nicer to your legs and feet, since heavier boards tend to wear down your legs faster, tiring you out a lot sooner than with a lighter board. The construction of this board makes it easy to feel like your flying across the slopes effortlessly. One of the reasons this board is able to handle speed and turns is due to its lightweight design. For those speed demons on the slopes, the Yup snowboard is a perfect choice. You’ll beat your friends to the end of the slopes, whether it’s on powder or a rougher terrain. The construction of the base with the triaxial glass and the carbon stringers ensure this board will have you flying down the mountain. The Signal Yup Snowboard is also incredibly fast. A responsive board is key to perfecting manoeuvres both basic and advanced and this board is ready no matter where you take it. With this board you’ll be able to maneuver both hard and softer turns, allowing you to play with both styles.Įspecially on uneven terrain, you will be able to dodge and dart around the bumps quickly and effortlessly. When you’re performing a trick, it will keep up with your demands.Įven more importantly, if you’re off-piste and come across a group of trees (or another snowboarder is coming toward you), the Yup board guarantees you can easily turn and avoid disaster, with quick response time and smooth transitions. It responds well to your movements and is incredibly precise. Testers also mentioned how well the Signal Yup Snowboard is at turns. It could lead to a nasty fall if you aren’t!Ī stable board gives riders that extra bit of confidence they need to perfect their skills and dominate the trails without worry of wiping out. The sidewalls and the camber profile help ensure that even on the most uneven terrain, you feel stable and in control.Įspecially when you’re doing jumps, turns, or tricks, you want to be sure of your board’s stability. ![]() There’s no worry of feeling wobbly or insecure on this board. Quite a few users mentioned how stable the Yup snowboard is. Signal Yup Snowboard Review The Positives The wood core ensures you instead feel like you’re floating on a cloud. ![]() When you’re going down a mountain or just gliding across the slopes, you don’t want to feel like there are weights on your feet. This makes the board both lightweight and durable. The core of the Yup is designed as a mixture of Paulownia and Poplar wood. When you’re making wide turns, or doing jumps and landing hard on the snow, you want your board to be durable with solid edge grip, so you don’t end up wiping out on the slopes. The ABS/TPU makes the sidewalls stiff, provide better edge grip, and give the whole board enhanced durability. The sidewalls on the Yup snowboard are crafted from premium ABS/TPU. The fiberglass makes the board more responsive and precise, while the carbon stringer makes the board stiffer and very damp. The glass on the base of the board is crafted from triaxial fiberglass and dual carbon stringers. This makes the board faster and more durable. What does this mean for you? It’s made of ultra-high molecular weight polyethylene that is modified with additives like Fluoro and Paraffin. The base of the Yup is a sintered ultra base. The rest of the board is flat against the snow to give you traction, as well as a bit of grip. What does this mean? The middle of the board is curved up toward where your feet would be placed, and the tip and the tail (or ends) of the board are also slightly curved up. The Signal Yup Snowboard has a traditional camber profile. Signal Yup Snowboard: The Details In A Nutshell 3 Where to Buy the Signal Yup Snowboard. ![]() ![]() ![]() If you want to have a look at Woord’s Chrome Extension, click here.Text to speech software has revolutionized content consumption. Additionally, if you are doing something else, you can listen to any written materials read by fictional characters. ![]() This service will also help your child develop their listening and communication skills, as well as their pronounciation. Sign up here: If you want to listen to some audios before signing up, you can explore his Public Audio Library. You are free to replay the text as many times as you want. Simply type a word or phrase, or import any text, and you’re done.Ĭhoose a speech rate that is comfortable for you. How do you use Woord’s Free Online Reader? This text-to-speech service employs natural-sounding male or female premium voices. The text-to-speech reader also supports Italian, French, Chinese, Dutch, German, Hindi, Indonesian, Japanese, Korean, Polish, Portuguese, Russian, and Spanish voices. If you need to use this service, there are several accents available: Australian English, French, American English, French Canadian or Brazilian Portuguese. You can pick the speech rate to slow or speed up the voice, and you can replay the audio as many times as you like with this app. When doing anything else, you can listen to some written materials in authentic voices. This service can be used to improve your listening and speaking skills as well as your pronunciation. This text-to-speech service offers high-quality, natural-sounding male, female, and neutral voices. It’s great for e-learning, workshops, YouTube videos, and enhancing the website’s usability. You can easily turn your text into professional speech for free using premium voices with the Woord chrome extension. Hearing the errors through Woord is much simpler and less difficult for many people than remembering them through reading. Listening to a text read aloud in a natural voice is a relatively new and still developing technology. ![]() Many people find Woord’s text-to-speech software to be very useful in allowing people of all ages to follow the speech on the computer. Text to speech is getting more human-like with each passing day. This technology can be used to convert text to speech and communicate information, which is a fantastic way to improve productivity. TTS apps have a lot to give people all over the world, and there are a lot of cool apps out there that can assist us in a number of ways. However, you can do text to speech conversions much more easily with the help of some free chrome plugins. There are several online services for text to speech translations, but the process is tedious and some of these services require payment. So why don’t we close our eyes and listen with our ears to the 1500-word article? Text to speech is, in reality, improving by the day, with human-like voices. This platform is used by people of all ages, and it also allows someone else seeking easy access to digital content to use it.Ĭonverting text to speech and using this technology to read online articles is unquestionably beneficial in terms of increasing productivity. ![]() For example, text-to-speech is a common assistive technology in which a device or tablet reads out loud the words on the screen to the user. Google Chrome has a lot to give users all over the world, and there are a lot of great applications and plugins that can help us out in a variety of ways. Is There Any Chrome Extension for Text-To-Speech in 2021? ![]() ![]() ![]() CLICK HERE FOR RULESĪNY PERSON PARTICIPATING IN A RAFFLE MUST BE 18 OR OVER - NO EXCEPTIONS. The moderators do their best to protect the community, but the final burden of responsibility falls on the users to determine whether or not they want to participate in a raffle. Request Raffle Approval Submit Mod Approved Raffle Submit Mod Approved Raffle Submit RAFFLE ($300 or less) Raffle Tool by /u/BoyAndHisBlob Email Raffle Notification Tool Note: The invoke must be the ONLY thing posted in the comment. When your raffle is full, use the following syntax to invoke the bot in a top-level comment: /u/BotAndHisBoy X CLICK HERE TO SEE ACTIVE RAFFLES Upcoming Raffles It's free to use and offers discounted rates comparable to any paid shipping service available online plus has an excellent insurance provider. Lower shipping costs with Pirate Ship label generator. Guide To Raffling Off Your First Lego Set! Part 66956, Wedge 2x2 Pointed with Stud Notches: a.Welcome to lego_raffles! Before participating in any raffles, please read through the sidebar and take a look at our rules and instructions linked below! Wiki.LEGO® Star Wars review: 75274 TIE Fighter Pilot He.LEGO® Trolls World Tour review: 41258 Vibe City Co.LEGO® Chinese Festival 80106 & 80107: new parts &.What new LEGO® pieces were released in December 20.Inside the LEGO® Element Design department: Interv.LEGO® Technic review: 42125 Ferrari 488 GTE 'AF Co.Old Elementary: Eero Okkonen's gentle LEGO® curves.LEGO® Ideas parts review: 21325 Medieval Blacksmith.LEGO® Ideas set review: 21325 Medieval Blacksmith.LEGO® 71741 NINJAGO® City Gardens interview: desig.LEGO® Super Mario review: 71380 Master Your Advent.LEGO® Disney review & MOCs: 43187 Rapunzel’s Tower.Which LEGO® sets have the most new parts? December.(CW: HP) LEGO® 75979 Hedwig: Professor's "One Set".It was the first fan-designed official LEGO set in fact, and sold as ‘My Own Creation’ way back in 2002.Īlthough that’s not quite as far back as Medieval times, if we look at what LEGO has done with 21325 in comparison to 3739 we can see just how far the medium has come in the last two decades. Seeing how the set promoted imaginative play in my children was a joy and further welded my opinion that this truly is a great set.īriefly mentioned in the instructions is 3739 Blacksmith Shop, a model that was also originally conceived by a fan, Daniel Siskind. My kids (7 and 9) both loved playing with the set once it was built, and spent an hour after our home learning commitments creating a complex story. I anticipate being able to easily make a variety of other buildings from this. I’ve a feeling it’ll be a great start for building your own Medieval village too. 21325 Medieval Blacksmith is packed full of good, useful pieces (some in great quantities) and without a vast number of small pieces bumping up the part count, if nothing else this represents a brilliant parts pack. There are a total of 2164 pieces here and for 149.99 USD/ 199.99 CAD/ 146.99 EUR/ 134.99 GBP/ 249.99 AUD, I think that’s astounding value. The overall footprint just about fits onto a 32 x 32 baseplate, which means anyone that wants to might be able (with a little trial and error) to integrate this into a modular display! It’s a fairly straightforward build throughout, but a really enjoyable journey nonetheless. "Ferrous enough, thanks for the unrequested lesson," you might say, but what about the LEGO? The build peining is when you want the metal to move in a certain direction, or all directions.upsetting is when you are broadening an end, adding mass.drawing out, as the name suggests, is a process of lengthening the piece of metal.There are three basic ways to shape the metal: Once you’ve mastered the three Hs, you’re ready to move on to Shaping. Hammers and anvils come in all sorts of shapes and sizes too. Something to hit with, and something hard and flat to hit on, would also be essential. Hitting: Accuracy is far more important than force. ![]() To adequately hold the workpiece still, you will need tongs (of which there are a vast array), clamps, vises and jigs. Holding: Once hot, if you’re unable to hold it, there’s no way you’ll be able to hit it.Heating: First the metal needs to be heated fairly precisely to the correct temperature for this you’ll probably need a forge (a hearth or stone-lined furnace). ![]() ![]() ![]() That being said, some employers may prefer candidates who have completed a certified training program. In short, no certification is required to become a medical scribe. With that being said, many facilities will provide training on their specific EHR system before expected you to be an expert. Finally, computer literacy is extremely important since you will be spending most of your day inputting data into an EHR. Keeping a professional appearance is also important since you will be representing the facility you work for. Obviously, strong written and verbal communication skills are key since you will be interacting with patients, physicians, and other staff members on a daily basis. However, there are some recommended qualifications that will make you a more attractive candidate. There is no one size fits all when it comes to becoming a medical scribe. ![]() In some cases, the scribe may also act as a liaison between the patient and physician outside of scheduled appointments if questions or concerns arise. This includes coordinating follow up appointments and referrals to specialists. Schedule management may also be a responsibility of the scribe. ![]() Recording physician-patient interactions That being said, there are some general duties that remain constant. The Job DescriptionĪ medical scribe’s responsibilities will vary depending on the state they work in and the specific facility’s policies. This position has become increasingly popular in recent years as physicians are being pushed to see more patients and have less time for documentation. Most often, the scribe will be inputting information into the patient’s electronic health record (EHR). A medical scribe is a person who works alongside a physician in order to documentation of patient visits, procedures, etc. You may have heard the term “medical scribe” or “clinical scribe” being thrown around and are wondering what exactly this job entails. ![]() ![]() The heartwood of maple, meanwhile, is characterized by its brownish-red coloring. ![]() While most hardwood species are sourced from the heartwood for its high-quality lumber, quality wood for furniture is actually sourced from maple sapwood with a fine, light, creamy color. The raw look also works with a minimalist style, providing an interesting contrast to a whitewashed backdrop. Without stains or paint, the natural look of maple kitchen cabinets makes them a great option for a rustic, country, or industrialist-themed kitchen. Quick Look – Types of Maple Wood Cabinets Maple wood furniture has also become widely known in overseas markets as it is regarded as a high-grade hardwood. Moreover, maple wood has increased in popularity as a result of its sustainable development. It is also renowned for its fine and very straight grain. ![]() Maple wood was frequently used in cabinet building, especially in the 20th century. Maple wood is popular among professional cabinetry makers, woodworkers, furniture manufacturers, and even DIY wood enthusiasts due to its creamy yet light shade and smooth texture. ![]() Maple wood is obtained from deciduous maple trees, of which there are over 200 various species in Europe, Asia, and North America. Maple wood is considered one of the most well-known woods in the industry, noted for its longevity, visual appeal, hardness, and ability to stain well. Maple Kitchen Cabinet Durability & Hardness.What Kitchen Designs Go With Maple Cabinets?. ![]() ![]() ![]() Warning - California Prop 65: Some products on this site can expose you to chemicals including Chloroprene, which is known to the state of California to cause cancer and birth defects or reproductive harm. This wonderful scuba and snorkeling set is PERFECT for taking on vacation or to your favorite local dive spot. 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